Coding

Part:BBa_K1903005:Design

Designed by: Sofía Vieto Fonseca   Group: iGEM16_TEC-Costa_Rica   (2016-10-14)


Split TEV N-IntN DnaE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 316


Design Notes

This parts is composed of a split TEV N terminal and the split intein N terminal from Npu DnaE fused together. These protein are expressed together and will separate from each other in the presence of the other split Intein C from Npu DnaE BBa_K1903008. Finally we also added the Double terminator BBa_B0015.

Source

This part was based on the following BioBricks: N terminal half of TEV BBa_K1159101 and NpuDnaE N-Intein BBa_K1362400.

References